In the Drosophila experiment you carried out, from which organisms

Question
1.Drosophila genes

(Points: 10)

In the Drosophila experiment you carried out, from which organisms were the following genetic elements derived?

Column preview

UAS promoter

yeast

Beta-galactosidase

bacteria

Gal-4

drosophila

Matching pairs

UAS promoter

Beta-galactosidase

Gal-4

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2.Lac operon 1

(Points: 10)

Imagine that in a e.coli culture that was growing with glucose as its nutrient source you could artificially increase cAMP levels (which are normally associated with starvation). What would happen to the expression of beta-galactosidase?

1. It would be activated because the cAMP binding protein CAP would bind to the beta-galactosidase promoter

2. It would be reduced

3. It would remain at the same relatively high level

4. It would remain at a very low level

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3.Gal4 1

(Points: 10)

Imagine you had these two strains of drosophila:
AA= contains the gene for Gal 4 and a UAS upstream of alcohol dehydrogenase

BB= has a UAS upstream of Beta- Galactosidase

What would you expect to be able to detect in the flies from the following crosses:

Column preview

AA X AA

a. Alcohol dehydrogenase

BB x BB

b. Nothing

AA X BB

c. Alcohol dehydrogenase and beta-galactosidase

d. Beta-galactosidase

Matching pairs

AA X AA

BB x BB

AA X BB

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4.tRNA 2

(Points: 10)

Which of the following stabilises the structure of tRNAs?

1. Binding to the correct amino acid

2. interaction with mRNA

3. binding to the ribosome

4. internal double stranded regions

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5.Wobble

(Points: 10)

According to the Crick’s wobble hypothesis what bases on the anticodon’s 1st position can bind to the codon 3rd position?

Column preview

C

C or U

A

U,C or A

U

U

G

G

I

A or G

Matching pairs

C

A

U

G

I

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6.Shine Delgrano sequence

(Points: 10)

Between which nucleic acids does the Shine Dalgrano sequence stabilises binding?

1. mRNA and tRNA

2. mRNA and small subunit tRNA

3. tRNA and large subunit of rRNA

4. tRNA and small subunit of rRNA

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7.signal peptide

(Points: 10)

Which of the following proteins are least likely to contain a signal peptide?

1. Cytosolic proteins

2. Nuclear Proteins

3. Secretory proteins

4. Membrane proteins

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8.Protein modificatons

(Points: 10)

What are the main roles of the following post-translation modifications of proteins

Column preview

Phosphorylaton

Activation/inactivation

Glycosidation

Degradation

Acylation

Association with membrane

Ubiquitinatio

Cell-cell recognition

Matching pairs

Phosphorylaton

Glycosidation

Acylation

Ubiquitinatio

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9.cloning

(Points: 10)

One of the advantages of expressing eukaryotic proteins in bacteria is that they are faithfully post-translationaly modified.

True
False

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10.chaperons

(Points: 10)

The structure and mechanism of action of chaperon proteins are conserved between prokaryotes and eukaryotes

True
False

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